朱本伟，教授/硕士生导师

联系方式

办公地点：生工楼A2-405

电话：025-58139419

邮箱：zhubenwei@njtech.edu.cn

教育/工作经历

2012.09-2015.06:  中国科学院大学，大连化学物理研究所，生物化工，博士

2015.09-2016.03：南京大学，化学化工学院，助理研究员/博士后

2020.12-2021.12：北海道大学，水产学部，访问研究员

2022.12-至今：中国科学院海洋研究所，海洋生物学流动站，博士后（在职）

2016.04-至今：365官方游戏官网(中国)有限公司，博士后、讲师、副教授、教授

奖励与荣誉

入选江苏省“双创人才”科技副总，曾获得江苏省海洋学会科技二等奖、江苏省高校教师教学创新大赛二等奖、365官方游戏官网晨星奖教金等荣誉。兼任江苏省海洋学会海洋生物资源开发专业委员会委员，河南省特色微生物资源开发与利用工程技术中心兼职教授，国家自然科学基金评审专家。

研究方向

1.海洋多糖降解酶的挖掘、筛选、表征、结构与功能研究；

2.海洋功能寡糖的高效绿色制备、分离纯化、活性评价与规范化应用；

3.海洋生物资源（海洋藻类资源）的高效开发利用。

主要学术成绩

近5年在Critical Reviews in Food Science and Nutrition、Applied Microbiology and Biotechnology、Biotechnology for Biofuels、Critical Reviews in Biotechnology、Glycobiology、Intentional Journal of Biological Macromolecules、Algal Research等主流学术期刊发表等国际著名期刊上发表论文50余篇。授权中国发明专利3项。主编普通高等教育十三五规划教材《海洋酶工程》1部、主编普通高等教育十四五规划教材《海洋酶工程实验教程》1部，主持企业横向课题多项，多项专利实现企业转化。

基金项目

1、  江苏省重点研发计划（现代农业）面上项目:食用菌菌渣资源化利用关键技术研究，主持，在研。

2、  江苏省海洋生物产业现状与发展路径研究，江苏省科技智库青年人才项目，主持，在研。

3、农业部海藻类肥料重点实验室开发基金：半理性设计用于定向改造新型褐藻胶裂解酶制备特定聚合度寡糖，主持，在研。

代表性论文

[1]       Benwei Zhu*, Fang Ni, Qiang Xiong, Zhong Yao*. Marine oligosaccharides originated from seaweeds: Source, preparation, structure, physiological activity and applications. Critical Reviews in Food Science and Nutrition (SCI 1区，影响因子：11.208), 2021, 61(1): 60-74.

[2]       Shengsheng Cao, Li Li, Qian Li, Ling Jiang, Benwei Zhu* & Zhong Yao. A novel alginate lyase and its domain functions for the preparation of unsaturated monosaccharides. Applied Microbiology and Biotechnology (SCI 2区, 影响因子：5.560), 2023, Doi: 10.1007/s00253-023-12424-4.

[3]       Tiancheng Tang, Shengsheng Cao, Qian Li, Benwei Zhu*. Ulvan polysaccharide-degrading enzymes: An updated and comprehensive review of sources category, property, structure, and applications of ulvan lyases, Algal Research (SCI 2区，影响因子：5.276) , 2021, 60:102477.

[4]       Ling Zheng, Yinxiao Xu, Qian Li, Benwei Zhu*. Pectinolytic lyases: a comprehensive review of sources, category, property, structure, and catalytic mechanism of pectate lyases and pectin lyases, Bioresources and Bioprocessing (SCI 2区，影响因子：4.983), 2021, 8:79

[5]       Ling Zheng, Zilong Guo, Shengsheng Cao, Benwei Zhu*. Elucidating the degradation pattern of a new cold‑tolerant pectate lyase used for efficient preparation of pectin oligosaccharides. Bioresources and Bioprocessing (SCI 2区，影响因子：4.983), 2021, 8:121

[6]       Zilong Guo, Yanshang Wei, Yu Zhang, Yinxiao Xu, Ling Zheng, Benwei Zhu*, Zhong Yao. Carrageenan Oligosaccharides: A Comprehensive Review of Preparation, Isolation, Purification, Structure, Biological activities and Applications. Algal Research (SCI 2区，影响因子：5.276) , 2021, 60: 102593.

[7]       Shengsheng Cao, Qian Li, Yinxiao Xu, Tiancheng Tang, Limin Ning, Benwei Zhu*.Evolving strategies for marine enzyme engineering: recent advances on the molecular modification of alginate lyase. Marine Life Science & Technology (新增SCI，影响因子5.000), 2021, Doi: 10.1007/s42995-021-00122-x

[8]       Qian Li#, Ling Zheng#, Zilong Guo, Tiancheng Tang, Benwei Zhu*. Alginate degrading enzymes: An updated comprehensive review of the structure, catalytic mechanism, modification method and applications of alginate lyases. Critical Reviews in Biotechnology (SCI 1区，影响因子：9.062), 2021, doi: 10.1080/07388551.2021.1898330.

[9]       Benwei Zhu^#*, Fang Ni^#, Yun Sun, Limin Ning, Zhong Yao*. Elucidation of degrading pattern and substrate recognition of a novel bifunctional alginate lyase from Flammeovirga sp. NJ-04 and its use for preparation alginate oligosaccharides. Biotechnology for Biofuels (SCI 1区，影响因子：7.670), 2019, 12:13.

[10]    Benwei Zhu, Kuikui Li, Wenxia Wang, Limin Ning, Haidong Tan, Xiaoming Zhao, Heng Yin. Preparation of trisaccharides from alginate by a novel alginate lyase Alg7A from marine bacterium Vibrio sp. W13. International Journal of Biological Macromolecules (SCI 2区，影响因子：8.025), 2019, 139: 879-885

[11]    Fu Hu, Qian Li, Benwei Zhu*, Fang Ni, Yun Sun, Zhong Yao. Effects of module truncation on biochemical characteristics and products distribution of a new alginate lyase with two catalytic modules. Glycobiology (SCI 2区，影响因子：5.954), 2019, 29(12):876-884. 

[12]    Fu Hu#, Shengsheng Cao#, Qian Li, Benwei Zhu*, Zhong Yao. Construction and biochemical characterization of a novel hybrid alginate lyase with high activity by module recombination to prepare alginate oligosaccharides. International Journal of Biological Macromolecules (SCI 2区，影响因子：8.025), 2021, 166: 1272-1279

[13]    Qian Li, Fu Hu, Mingyang Wang, Benwei Zhu*, Fang Ni, Zhong Yao. Elucidation of degradation pattern and immobilization of a novel alginate lyase for preparation of alginate oligosaccharides. International Journal of Biological Macromolecules (SCI 2区，影响因子：8.025), 2020, 146: 579-587.

[14]    Fu Hu, Benwei Zhu*, Qian Li, Heng Yin, Yun Sun, Zhong Yao, Dengming Ming. Elucidation of a Unique Pattern and the Role of Carbohydrate Binding Module of an Alginate Lyase. Marine Drugs (SCI 2区，影响因子：6.085), 2020, 18(1):32.

[15]    Qian Li, Fu Hu, Benwei Zhu*, Fang Ni, Zhong Yao. Insights into Ulvan lyase: review of source, biochemical characteristics, structure and catalytic mechanism. Critical Reviews in Biotechnology (SCI 1区，影响因子：9.062), 2020, 40(3): 432-441. 

[16]    Benwei Zhu*, Fang Ni, Yun Sun, Xianyu Zhu, Heng Yin, Zhong Yao*, Yuguang Du. Insight into Carrageenases: Major Review of Category, Property, Structure and Applications. Critical Reviews in Biotechnology (SCI 1区，影响因子：9.062), 2018, 38: 1261-1276. 

[17]    Benwei Zhu*, Fu Hu, Heng Yuan, Yun Sun, Zhong Sun, Zhong Yao*. Biochemical Characterization and Degradation Pattern of a Unique pH-stable polyM-specific Alginate Lyase from Newly Isolated Serratia marcescens NJ-07. Marine Drugs (SCI 2区，影响因子：6.085), 2018, 16:129. 

[18]    Qian Li, Fu Hu, Benwei Zhu*, Yun Sun, Zhong Yao. Biochemical Characterization and Elucidation of Action Pattern of a Novel Polysaccharide Lyase 6 Family Alginate Lyase from Marine Bacterium Flammeovirga sp. NJ-04, Marine Drugs (SCI 2区，影响因子：6.085), 2019, 17(6), 323. 

[19]    Benwei Zhu*, Yun Sun, Fang Ni, Limin Ning, Zhong Yao*. Characterization of a new endo-type alginate lyase from Vibrio sp. NJU-03. International Journal of Biological Macromolecules (SCI 2区，影响因子：8.025), 2018, 108: 1140-1147.

[20]    Benwei Zhu*, Fang Ni, Limin Ning, Yun Sun, Zhong Yao*. Cloning and characterization of a new pH-stable alginate lyase with high salt tolerance from marine Vibrio sp. NJ-04. International Journal of Biological Macromolecules (SCI 2区，影响因子：8.025), 2018, 115: 1063-1070. 

[21]    Benwei Zhu*, Fang Ni, Yun Sun, Zhong Yao, Expression and Characterization of a New Heat-stable Endo-type Alginate Lyase from Deep-sea Bacterium Flammeovirga sp. NJ-04. Extremophiles (SCI 3区，影响因子：3.035). 2017, 21: 1027–1036. 

[22]    Benwei Zhu#*, Qiang Xiong#, Fang Ni, Yun Sun, Zhong Yao, High-level expression and characterization of a new -carrageenase from marine bacterium Pedobacter hainanensis NJ-02. Letters in Applied Microbiology (SCI 3区，影响因子：2.813), 2018, 66(5):409-415.

[23]    Benwei Zhu, Limin Ning*, Yun Sun, Zhong Yao, Cloning and biochemical characterization of a novel κ-carrageenase from newly isolated marine bacterium Pedobacter hainanensis NJ-02. International Journal of Biological Macromolecules (SCI 2区，影响因子：8.025). 2017, 108:1331-1338.

[24]    Zuochen Yu^#, Benwei Zhu^#, Wenxia Wang, Haidong Tan, Heng Yin. Characterization of a new oligoalginate lyase from marine bacterium Vibrio sp. International Journal of Biological Macromolecules (SCI 2区，影响因子：8.025). 2018, 112:937-942. 

[25]    Benwei Zhu, Meijuan Chen, Heng Yin, Yuguang Du, Limin Ning. Enzymatic Hydrolysis of Alginate to Produce Oligosaccharides by a New Purified Endo-Type Alginate Lyase. Marine Drugs (SCI 2区，影响因子：6.085), 2016, 14(6), 108. 

[26]    Benwei Zhu, Haidong Tan, Yuqin Qin, Qingsong Xu, Yuguang Du, Heng Yin. Characterization of a new endo-type alginate lyase from Vibrio sp. W13. International Journal of Biological Macromolecules (SCI 2区，影响因子：8.025). 2015, 75:330-337. 

[27]    Benwei Zhu, Limin Ning. Purification and Characterization of a New κ-Carrageenase from the Marine Bacterium Vibrio sp. NJ-2. Journal of Microbiology and Biotechnology (SCI 3区，影响因子：3.277) 2016; 26(2): 255-262.

[28]    Benwei Zhu, Heng Yin. Alginate lyase: Review of major sources and classification, properties, structure-function analysis and applications. Bioengineered (SCI 4区，影响因子：6.832), 2015, 6(3):125-131.

[29]    Benwei Zhu, Lishuxin Huang, Haidong Tan, Yuqi Qin, Yuguang Du, Heng Yin. Characterization of a new endo-type polyM-specific alginate lyase from Pseudomonas sp. Biotechnology Letters (SCI 4区，影响因子：2.716), 2015 37:409–415.

[30]    Shengsheng Cao, Li Li, Benwei Zhu*, Zhong Yao. Biochemical Characterization and Elucidation of the Hybrid Action Mode of a New Psychrophilic and Cold-Tolerant Alginate Lyase for Efficient Preparation of Alginate Oligosaccharides, Marine Drugs(SCI 2区，影响因子：6.085), 2022, 20(8).

[31]    Yinxiao Xu, Shengsheng Cao, Li Li, Benwei Zhu*, Zhong Yao. An updated comprehensive review of advances on structural features, catalytic mechanisms, modification methods and applications of chitosanases, Process Biochemistry(SCI 3区，影响因子：4.885), 2022, 118:263-273.

[32]    Benwei Zhu*, Yun Sun, Fang Ni, Limin Ning, Zhong Yao. Characterization of a new endo-type alginate lyase from Vibrio sp. NJU-03, International Journal of Biological Macromolecules(SCI 2区，影响因子：8.025), 2017, 108: 1140-1147.

[33]    Benwei Zhu, Lishuxin Huang, Haidong Tan, Yuqi Qin, Yuguang Du, Heng Yin. Characterization of a new endo-type polyM-specific alginate lyase from Pseudomonas sp. Biotechnology Letters (SCI 3区，影响因子：2.716), 2015, 37, 409–41536.

[34]    Limin Ning, Zhong Yao, Benwei Zhu*. Ulva (Enteromorpha) Polysaccharides and Oligosaccharides: A Potential Functional Food Source from Green-Tide-Forming Macroalgae, Marine Drugs (SCI 2区，影响因子：6.085), 2022, 20(3), 202

[35]    Yinxiao Xu, Hui Wang, Benwei Zhu* & Zhong Yao. Biochemical characterization and elucidation action mode of a new endolytic chitosanase for efficient preparation of chitosan oligosaccharides, Biomass Conversion and Biorefinery (SCI2区，影响因子：4.050), 2023, Doi: 10.1007/s13399-023-03870-1.

[36]    Shengsheng Cao, Li Li, Benwei Zhu*, Zhong Yao. Alginate modifying enzymes: An updated comprehensive review of the mannuronan C5-epimerases, Algal Research (SCI2区，影响因子：5.276), 2023, 69: 102952.

[37]    Tiancheng Tang, Benwei Zhu*, Zhong Yao. Biochemical characterization and elucidation the action mode of a new PL25 family ulvan lyase from marine bacterium Alteromonas sp. TK-45 (2), Algal Research (SCI2区，影响因子：5.276), 2022, 67: 102854.

[38]    Shengsheng Cao, Li Li, Benwei Zhu*, Zhong Yao. Biochemical Characterization and Elucidation of the Hybrid Action Mode of a New Psychrophilic and Cold-Tolerant Alginate Lyase for Efficient Preparation of Alginate Oligosaccharides, Marine Drugs (SCI 2区，影响因子：6.085), 2022, 20(8), 506

[39]    Cheng Chen, Shengsheng Cao, Benwei Zhu*, Ling Jiang, Zhong Yao. Biochemical characterization and elucidation the degradation pattern of a new cold-adapted and Ca2+ activated alginate lyase for efficient preparation of alginate oligosaccharides, Enzyme and Microbial Technology (SCI2区，影响因子：3.705),2023, 162, 110146

[40]    Ling Zheng, Zilong Guo, Yinxiao Xu, Benwei Zhu*, Zhong Yao. Biochemical characterization and immobilization of a novel pectate lyase ErPL2 for efficient preparation of pectin oligosaccharides, International Journal of Biological Macromolecules(SCI 2区，影响因子：8.025), 2022, 204: 532-539.

[41]   李谦，胡富，宁利敏*，朱本伟*，倪芳，姚忠。褐藻胶裂解酶的结构及催化机制研究进展，生物加工过程（科技核心），2020, 18(5)：592-598.

[42]   胡富，李谦，朱本伟*，宁利敏，姚忠，孙芸，杜昱光。石莼多糖裂解酶的研究进展，中国生物工程杂志（中文核心），2019, 39(8)：104-113.

[43]   陈旭，姚忠，袁玮康，孙芸，朱本伟*。海洋细菌Pedobacter sp.NJ-02 产κ-卡拉胶酶的发酵条件优化，现代食品科技（中文核心），2017, 33(12)：155-161.

[44]   宁利敏，朱玉翠，于光，曹声生，朱本伟*。几种海洋功能寡糖的结构、制备、活性与应用研究进展，海洋科学（中文核心），2021，45(2): 144-151。

[45]   曹声生，朱本伟*，高翔，王剑朋，杜媛媛，周婉婷，姜进举，周明柱，姜帅。岩藻寡糖的制备及其抗氧化与益生活性，生物加工过程（科技核心），2021,19(1)：33-38。

[46]   郭子龙，唐天城，徐寅啸，朱本伟*，姚忠, 宁利敏。卡拉胶酶的来源、性质、结构与应用研究进展，生物加工过程（科技核心），2022, 20(05):476-489。

[47]   郑玲，李谦，徐寅啸，宁利敏，朱本伟*，姚忠。微生物来源的果胶相关裂解酶的研究进展，生物加工过程（科技核心），2022, 20(06): 608-620+694。

[48]   曹声生，徐寅啸，唐天城，宁利敏，朱本伟*，倪芳，姚忠。褐藻胶裂解酶的分子改造研究进展，生物加工过程（科技核心），2023-02-22，录用定稿，网络首发。

[49]   徐寅啸，曹声生，唐天城，宁利敏，朱本伟*，倪芳，姚忠。壳聚糖酶的研究进展，生物加工过程（科技核心），2023,21(01)：15-24+38。

[50]   李丽，宁利敏，姚忠，徐虹，朱本伟*，褐藻胶多糖分子修饰酶的研究进展，食品与生物技术学报（中文核心），2023,42(01):18-39。

[51]   魏衍尚，宁利敏，姚忠，朱本伟*，卡拉胶多糖的分子修饰：卡拉胶酶和硫酸化酶的研究进展，食品与生物技术学报（中文核心），2022,41(10):17-36。

[52]   唐天成，朱本伟*，熊强，姚忠，孙芸，石莼多糖结构、制备及降解的研究进展，现代食品科技（中文核心），2023，Doi:10.13982/j.mfst.1673-9078.2023.5.0648。

[53]   宁利敏，姚忠，朱本伟*，孙芸。浒苔多糖的结构、制备与降解研究进展，现代食品科技（中文核心），2022,38(06)：343-353.

授权专利及科研获奖

[1] 朱本伟，李谦，姚忠，熊强，孙芸。褐藻胶裂解酶AlyPL17、截短体及其应用, 专利号：ZL202110690279.0 (已授权)

[2]朱本伟，倪芳，胡富，姚忠，孙芸，李谦。一种截短的褐藻胶裂解酶Aly7B-CDII基因及其应用, 专利号：ZL201910854192.5 (已授权)

[3]朱本伟，宁利敏，姚忠，熊强，倪芳，孙芸。海藻多糖降解工具酶的高效制备与应用技术，江苏省海洋学会科学技术二等奖，2021年12月。

[4]朱本伟，宁利敏，倪芳，姚忠，李丽，魏衍尚，王辉，李辰。江苏省海藻功能寡糖库的构建及应用技术，江苏省“科创江苏”创新创业大赛农业科技领域一等奖，2022年12月。